Perfusion systems

Our perfusion systems are designed for pharmacological researchers investigating small animals’ liver, kidney or mesenteric beds, in health and disease, or following drug challenges.

Ex vivo perfusion ensures highly reproducible experimental conditions, with no confounding effect from other organs or neuro-hormonal substances.

Our systems provide heated, oxygenated and nutrient-rich perfusate to the organs to ensure their survival during the study.

TYPICAL APPLICATIONS: Kidney transplantation surgery, Kidney secretory function, Renal metabolism, Liver malignancies, Liver fibrosis, Hepatotoxicity

Benefits

1

Highly reproducible

2

Constant pressure or constant flow

3

Perfect temperature monitoring

Description

Liver / kidney / Mesenteric beds perfusion

Especially designed for mice and rats liver, kidney or mesenteric beds perfusion, this system is perfectly thermostated. Indeed, maintaining a constant temperature is essential in order to avoid any discrepancies in the results or to conduct ischemia reperfusion experiments.

The temperature is kept constant, while oxygen and nutrients are provided by perfusing the organ at a constant flow or a constant pressure rate, through the artery.

The moist chamber: Including base and cover, it houses the organ to be perfused and protect it from heat-loss.

Perfusion system: To provide oxygen and nutrients to the isolated organ, it includes a fritted glass, a perfusate reservoir, a peristaltic pump, a pressure/flow regulator, holders and cannulas for veina and artery perfusion.

Heating system: Made of a heat exchanger and a heater/pump, it maintains the liver or kidney at the right temperature (usually 37°C).

Heated water passes through the moist chamber, bathing the heat exchanger through which the perfusate flows. In this way, heat is transferred from the heated water to the perfusate.

Measurement and acquisition system: transducers, usbACQ interface box and IOX2 software allow to acquire perfusion flow and pressure.


4 channels mesenteric beds perfusion

Designed for the perfusion of up to 4 mesenteric beds, this systems allows the acquisition of pressure, measured just upstream of the cannula, reflecting changes in vascular tone.

Each organ lies on a stainless steel grid and bathes in a buffer solution.

The organ is perfused at a constant flow rate (from 5 to 20 ml/min) via its artery. Heated water is circulated continually through the space behind the bath walls to maintain the buffer solution at the right temperature. Buffer flow rate is 5 to 10 times greater (maximum 150ml/min.) than perfusion flow in order for perfusion to fully infuse bath liquid.

4 organ baths:  Equipped with a stainless steel grid, organ bed height and bath volume are adjustable

3 peristaltic pumps: To provide mesenteric perfusion, bath perfusion and baths overflow (to aspirate excess of liquid going out of the bath due to mesenteric perfu­sion and bath perfusion)

A heating system: To keep the temperature of the perfusate constant.

Carbogen supply: Carbogen is released directly into the buffer solution.

Several small cannulas available to suit different artery sizes.

Data acquisition system: blood pressure transducer, usbAMP amplifier and IOX2 software for pressure acquisition

A stimulator: if electrical stimulation is required

Services

Support

Training

Validation

Data analysis

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