isolated HEART system
The isolated heart system is a versatile solution for pharmacological researchers investigating mice, rats, guinea-pigs or rabbits hearts, in health and disease, or following drug challenges.
Our isolatedHEART system provides highly reproducible experimental conditions to ensure survival of the isolated heart in Langendorff or Working heart perfusion mode, allowing the simultaneous assessment of
cardiac electrophysiological and mechanical parameters. Inotropic and chronotropic response in the isolated heart can be easily investigated.
A special care has been taken to insure low dead volumes and good temperature regulation (this is a key point, especially for study on Murine heart).
Fully integrated, this apparatus offers 3 perfusion modes and the ability to perfuse up to 3 physiological liquids.
Along with IOX2 software, it permits the acquisition of cardiac parameters through flow probes, pressure catheters or ECG electrodes.
Benefits
Modular
Easy to use
Real-time analysis
Description
Versatile, flexible and easy-to-use system
Fully integrated, with all tubing clearly organized, yet easily accessible, this apparatus has a spacious heart chamber, equipped with heated walls and floor and a wide opening through sliding door.
The temperature is kept constant at 37°C, while oxygen and nutrients are provided by perfusing the heart.
The same system can easily switch from Langendorff to working heart mode, by simply pressing a button, and offers:
- Up to 3 physiological perfusion liquids
- Temperature monitoring
- Optional electrical stimulation (pacing)
- Measurements of perfusion flow and pressures, LVP (by balloon or pressure sensor tip) and ECG
- 3 perfusion modes :
- Constant flow in Langendorff mode
- Constant pressure in Langendorff mode
- Constant pressure in atria, with post charge in aorta in Working Heart mode
Langendorff vs Working heart perfusion
In the Langendorff heart perfusion mode, the perfusate is pumped through the aorta towards the heart. It does not enter the left ventricle, but is diverted into the coronary arteries.
This mode permits the study of heart contraction, heart rate and vascular effects. It is ideal for the study of intraventricular pressure, cardiac contractility and relaxation, ECG and coronary flow.
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In the working heart perfusion mode, the flow of perfusate mimics the flow of blood in situ. The perfusate enters the left atrium via the pulmonary vein, is pumped into the left ventricle, from where it is pumped into the aorta, against a resistance that mimics the global resistance of the body.
As the name implies, this technique allows the heart to perform its physiological pumping action, i.e. it performs pressure/volume work. Therefore it provides a complete analysis of heart function and can be used for the study of cardiac metabolism, long-term pathology or ischemia-reperfusion.
Applications
Cardiomyopathy, Cardiac metabolism, Valvular heart disease, Ischemia-reperfusion, Pulmonary hypertension and more!
Related publications
Klotho protein contributes to cardioprotection during ischaemia/reperfusion injury
Agnieszka Olejnik et al, J Cell Mol Med, 2020
Assessment of Multiple Positive Controls in the Langendorff Isolated Heart Assay and How They Translate to Whole Animal
Tyler Ardrey et al, poster presented at the Safety Pharmacology Society, 2018
Study of Intrinsic Cardiac Dysfunction in Septic Shock Conditions by Isolated Working Heart: A Primary Approach before New Therapeutic Proposals?
Marine Ferron et al, SM Emergency Medicine and Critical Care , 2017, 1 (3)
PK and Cardiac Assessment in Rats Administered Doxorubicin
Kim A. Henderson et al, poster presented at SPS in Prague, 2015
Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury
Dariusz Bialy et al, BioMed Research International, 2014
Increased beta2-adrenoceptors in doxorubicin-induced cardiomyopathy in rat
Nolwenn Merlet et al, PLoS One, 2013
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